Supplementary Information Energy barriers and driving forces of tRNA translocation through the ribosome
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چکیده
Supplementary Note 1 (Methods) 18 1.1 General molecular dynamics setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 1.2 Models of the ribosome including tRNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 1.3 Refinement of the atomic models against cryo-EM maps . . . . . . . . . . . . . . . . . . . . . 19 1.4 Choice of models for simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 1.5 MD simulations of the refined models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 1.6 Cryo-EM analysis of global ribosome conformation . . . . . . . . . . . . . . . . . . . . . . . . 21
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